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| Status |
Public on Sep 30, 2019 |
| Title |
Pre-existent adult sox10+ cardiomyocytes contribute to myocardial regeneration in the zebrafish |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
During heart regeneration in the zebrafish, fibrotic tissue is replaced by newly formed cardiomyocytes derived from pre-existing ones. It is unclear whether the heart is comprised of several cardiomyocyte populations bearing different capacity to replace lost myocardium. Here, using sox10 genetic fate mapping, we identified a subset of pre-existent cardiomyocytes in the adult zebrafish heart with a distinct gene expression profile that expanded massively after cryoinjury. Genetic ablation of sox10+ cardiomyocytes severely impaired cardiac regeneration revealing that they play a crucial role for heart regeneration.
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| Overall design |
Adult zebrafish heart tg(sox10:CreERT2;vmhcl:loxP-tagBFP-loxP-mCherry-NTR) were disassociated and 20 CMs were FAC-sorted in single tubes for DESeq2 Library preparation
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| Contributor(s) |
Sande-Melon M, Ferreira-Francisco DM, Botos M, Mercader N |
| Citation(s) |
31644901 |
| |
| Submission date |
Jul 01, 2019 |
| Last update date |
Oct 29, 2019 |
| Contact name |
Nadia Mercader |
| E-mail(s) |
nmercader@cnic.es, nadia.mercader@unibe.ch
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| Organization name |
University of Bern
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| Street address |
Baltzerstrasse 2
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| City |
Bern |
| ZIP/Postal code |
3012 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL23274 |
Illumina HiSeq 3000 (Danio rerio) |
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| Samples (25)
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| Relations |
| BioProject |
PRJNA551951 |
| SRA |
SRP212668 |
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