NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE133571 Query DataSets for GSE133571
Status Public on Sep 30, 2019
Title Pre-existent adult sox10+ cardiomyocytes contribute to myocardial regeneration in the zebrafish 
Organism Danio rerio
Experiment type Expression profiling by high throughput sequencing
Summary During heart regeneration in the zebrafish, fibrotic tissue is replaced by newly formed cardiomyocytes derived from pre-existing ones. It is unclear whether the heart is comprised of several cardiomyocyte populations bearing different capacity to replace lost myocardium. Here, using sox10 genetic fate mapping, we identified a subset of pre-existent cardiomyocytes in the adult zebrafish heart with a distinct gene expression profile that expanded massively after cryoinjury. Genetic ablation of sox10+ cardiomyocytes severely impaired cardiac regeneration revealing that they play a crucial role for heart regeneration.
 
Overall design Adult zebrafish heart tg(sox10:CreERT2;vmhcl:loxP-tagBFP-loxP-mCherry-NTR) were disassociated and 20 CMs were FAC-sorted in single tubes for DESeq2 Library preparation
 
Contributor(s) Sande-Melon M, Ferreira-Francisco DM, Botos M, Mercader N
Citation(s) 31644901
Submission date Jul 01, 2019
Last update date Oct 29, 2019
Contact name Nadia Mercader
E-mail(s) nmercader@cnic.es, nadia.mercader@unibe.ch
Organization name University of Bern
Street address Baltzerstrasse 2
City Bern
ZIP/Postal code 3012
Country Switzerland
 
Platforms (1)
GPL23274 Illumina HiSeq 3000 (Danio rerio)
Samples (25)
GSM3912404 CICh1
GSM3912405 CICh17
GSM3912406 CICh33
Relations
BioProject PRJNA551951
SRA SRP212668

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE133571_RAW.tar 3.3 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap