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| Status |
Public on Mar 11, 2020 |
| Title |
CxxC Finger Protein 1-mediated Histone H3 Lysine-4 Trimethylation is Essential for Proper Meiotic Crossover Formation in Mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
CXXC finger protein 1 (Cfp1) is a DNA-binding component of the SETD1 methyltransferase complex, targets SETD1A/B to most CpG islands (CpGI), and mediates the generation of H3K4me3. Deficiency of CFP1 in mice leads to pre-implantation lethality. Previous data suggest an indispensable role of CFP1 in germ cell development and meiosis. However, it remains unclear if CFP1-mediated H3K4 trimethylation is also required for the earliest stages of meiosis in both male and female germ cells. Here, we revealed that Cxxc1 deletion caused a decrease of H3K4me3 levels in spermatocytes after the zygotene stage, impaired double strand breaks (DSBs) repairing, and crossover formation in meiotic prophase. As the results, Cxxc1-deleted spermatocytes failed to complete meiosis and were arrested at the meiosis II. ChIP-seq results revealed that H3K4me3 globally descreased at transcriptional start sites in Cxxc1-null spermatocytes at the leptotene/zygotene and pathytene stages.RNA-seq at different stages revealed an earlier expression of genes within the spermatogenesis pathway in Cxxc1-null spermatocytes. These results indicated that CFP1 is required for H3K4me3 accumulation at the gene promoters of male germ cells and play a key role in regulating programed gene expression that is essential for spermatogenesis.
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| Overall design |
We examined H3K4me3 and transcriptomes in WT and Cxxc1-null spermatocytes at different stages.
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| Contributor(s) |
Shen L |
| Citation(s) |
36396044 |
| Submission date |
Jul 01, 2019 |
| Last update date |
Sep 21, 2023 |
| Contact name |
Li Shen |
| E-mail(s) |
shenlab@zju.edu.cn
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| Phone |
86-0571-88981751
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| Organization name |
Life Sciences Institute, Zhejiang University
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| Lab |
Shenlab
|
| Street address |
866 Yuhangtang Road
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| City |
Hangzhou |
| State/province |
Zhejiang Province |
| ZIP/Postal code |
310058 |
| Country |
China |
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| Platforms (1) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA552071 |
| SRA |
SRP212713 |
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