Expression profiling by high throughput sequencing Other
Summary
The goal of this study was to position all transcripts extremities in two species of Cryptococcus using TSS-Seq and QuantSeq 3' mRNA-Seq when cells are grown under different conditions. We analysed also the level of expression of each genes in the same condition using the same cell sample. All these data have spiked in using a fixed quantity of S. cereviae cells added just before DNA and RNA extraction.
Overall design
total RNA and DNA were extracted different Cryptococcus wild type and mutant strains and sequenced. TSS and PAS were positioned and gene expression was evaluated.
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