|
| Status |
Public on Oct 31, 2008 |
| Title |
Effect of amino acid availability on vitamin B12 production in Lactobacillus reuteri |
| Organism |
Limosilactobacillus reuteri subsp. reuteri F275 |
| Experiment type |
Expression profiling by array
|
| Summary |
Recent functional genomics and genome-scale modeling approaches indicated that B12 production in Lactobacillus reuteri could be improved by medium optimization. Here we show that a series of systematic single amino acid omissions could significantly modulate the production of B12 from nearly undetectable levels (by isoleucine omission) to 20-fold higher than previously reported through omission of cysteine. We analyzed by cDNA microarray experiments the transcriptional response of L. reuteri to the medium lacking cysteine. These results supported the observed high B12 production and provided new avenues for future improvement of production of vitamin B12.
Keywords: cell type comparison
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| |
|
| Overall design |
loop design
|
| |
|
| Contributor(s) |
Santos F, Teusink B, Hugenholtz J, Molenaar D |
| Citation(s) |
19376900 |
| |
| Submission date |
Oct 29, 2008 |
| Last update date |
Mar 20, 2012 |
| Contact name |
Douwe Molenaar |
| E-mail(s) |
douwe.molenaar@falw.vu.nl
|
| Organization name |
Vrije Universiteit Amsterdam
|
| Department |
Systems Bioinformatics
|
| Street address |
De Boelelaan 1085
|
| City |
Amsterdam |
| ZIP/Postal code |
1081 HV |
| Country |
Netherlands |
| |
|
| Platforms (1) |
| GPL7505 |
Lactobacillus reuteri F275 44K 60-mer array, Version 1 |
|
| Samples (13)
|
|
| Relations |
| BioProject |
PRJNA109819 |
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