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Series GSE133943 Query DataSets for GSE133943
Status Public on Jul 09, 2019
Title SFRPs Are Biphasic Modulators of Wnt Signaling-elicited Cancer Stem Cell Properties beyond Extracellular Control
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Secreted frizzled-related proteins (SFRPs) are mainly known for their role as extracellular modulators and tumor suppressors that down-regulate Wnt signaling. Using the established (CRISPR/Cas9 targeting promoters of SFRPs and targeting SFRPs transcript) system, we find that nuclear SFRPs interact with β-catenin and either promote or suppress TCF4 recruitment. SFRPs bind with β-catenin on both their N and C termini, which the repressive effects caused by SFRP-β-catenin-N-terminus binding overpower the promoting effects of their binding at the C terminus. By high Wnt activity, β-catenin and SFRPs only bind with their C termini, which results in the up-regulation of β-catenin transcriptional activity and cancer stem cell (CSC)-related genes. Furthermore, we identify disulfide bonds of the CRD domain and two threonine phosphorylation events of the NTR domain of SFRPs that are essential for their role as biphasic modulators, suggesting that SFRPs are biphasic modulators of Wnt signaling-elicited CSC properties beyond extracellular control.
 
Overall design Total cellular RNA was extracted from HM20 Mock and SFRP1-5-expressing stable clones after treatment with Wnt3a-containing medium for 24 hours.
 
Contributor(s) Lee J
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Submission date Jul 08, 2019
Last update date Jul 11, 2019
Contact name Jia-Lin Lee
E-mail(s) jllee@life.nthu.edu.tw
Organization name National Tsing Hua University
Street address 101, Sec. 2, Kuang Fu Road
City Hsinchu
ZIP/Postal code 30013
Country Taiwan
 
Platforms (1)
GPL16951 Phalanx Human OneArray Ver. 6 Release 1
Samples (6)
GSM3930465 HM20 SFRP1 cells_Wnt3A treated 24h_rep1
GSM3930466 HM20 SFRP2 cells_Wnt3A treated 24h_rep1
GSM3930467 HM20 SFRP3 cells_Wnt3A treated 24h_rep1
Relations
BioProject PRJNA553187

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE133943_RAW.tar 9.5 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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