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| Status |
Public on Feb 28, 2020 |
| Title |
RNA-seq analysis of WT and HDAC1f/f HDAC2f/+ x CD4cre deficient activated helper T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
CD4+ T cells have the potential to differentiate into effector cells with cytotoxic activity (CD4+ CTLs), however molecular mechanisms regulating CD4+ CTL induction are poorly understood. By analyzing mice with graded deletion of Hdac1 and Hdac2 alleles in T cells, we observed an HDAC1-HDAC2 dose-dependent induction of CD4+ CTLs. A transcriptome analysis of activated CD4+ T cells carrying a combined deletion of two Hdac1 and one Hdac2 alleles (HDAC1f/f-HDAC2f/+) revealed enrichment of gene signatures similar to CD8+ T cells and CD4+ CTLs.
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| Overall design |
mRNA expression profiles of activated CD4+ T cells isolated from WT and HDAC1f/f HDAC2f/+ (CD4-cre) mice were generated by deep sequencing.
mRNA expression profiles of activated CD4+ T cells isolated from WT and HDAC1f/f (CD4-cre) mice were generated by deep sequencing.
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| Contributor(s) |
Preglej T, Goeschl L, Ellmeier W |
| Citation(s) |
32102981 |
| |
| Submission date |
Jul 16, 2019 |
| Last update date |
Mar 01, 2020 |
| Contact name |
Wilfried Ellmeier |
| E-mail(s) |
wilfried.ellmeier@meduniwien.ac.at
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| Organization name |
Medical University of Vienna
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| Department |
Institute of Immunology
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| Street address |
Lazarettgase 19
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| City |
Vienna |
| ZIP/Postal code |
1030 |
| Country |
Austria |
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| Platforms (2) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA554874 |
| SRA |
SRP214857 |
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