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Status |
Public on Jul 01, 2021 |
Title |
Transcriptomic profiling of circulating T follicular helper cells in kidney transplant recipients |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We compared the transcriptomic profile between patients without donor-specific HLA antibodies (DSA-), patients with donor-specific HLA antibodies without antibody-mediated rejection (DSA+ ABMR-) and patients with donor-specific HLA antibodies and antibody-mediated rejection (DSA+ ABMR+). We found that patient with DSA+ ABMR+ had a distinct transcriptomic profile compared to DSA- and DSA+ ABMR-, characterized by upregulation of genes encoding for germinal center T follicular helper cells function, T-B cell interaction and costimulatory molecules.
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Overall design |
We analysed N=3 samples per group of patients (DSA-, DSA+ABMR-, DSA+ ABMR+), without replicates
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Contributor(s) |
Louis K, Metes D, Chandran U, Chang A |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
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Submission date |
Jul 18, 2019 |
Last update date |
Jul 02, 2021 |
Contact name |
Uma Chandran |
E-mail(s) |
chandran@pitt.edu
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Phone |
412-648-9326
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Organization name |
University of Pittsburgh
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Department |
BioMedical Informatics
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Street address |
5150 Center Ave
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City |
Pittsburgh |
State/province |
PA |
ZIP/Postal code |
15232 |
Country |
USA |
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Platforms (1) |
GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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Samples (9)
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GSM3950597 |
RNA-seq circulating T follicular helper cells DSA- [1TFH_S6] |
GSM3950598 |
RNA-seq circulating T follicular helper cells DSA- [3TFH_S4] |
GSM3950599 |
RNA-seq circulating T follicular helper cells DSA- [4TFH_S17] |
GSM3950600 |
RNA-seq circulating T follicular helper cells DSA+ ABMR- [5TFH_S11] |
GSM3950601 |
RNA-seq circulating T follicular helper cells DSA+ ABMR- [6TFH_S10] |
GSM3950602 |
RNA-seq circulating T follicular helper cells DSA+ ABMR- [7TFH_S14] |
GSM3950603 |
RNA-seq circulating T follicular helper cells DSA+ ABMR+ [8TFH_S3] |
GSM3950604 |
RNA-seq circulating T follicular helper cells DSA+ ABMR+ [9TFH_S12] |
GSM3950605 |
RNA-seq circulating T follicular helper cells DSA+ ABMR+ [10TFH_S2] |
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Relations |
BioProject |
PRJNA555251 |
SRA |
SRP215220 |