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| Status |
Public on Jul 15, 2020 |
| Title |
Expression data of PRDM14 overexpression in WT, Suz12 KO and Ctbp1/2 DKO ESCs |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The pluripotency-associated transcriptional network is regulated by a core circuitry of transcription factors. The PR domain-containing protein, PRDM14, maintains pluripotency by activating and repressing transcription in a target gene-dependent manner. However, the mechanisms underlying dichotomic switching of PRDM14-mediated transcriptional control remains elusive. Here, we identified C-terminal binding protein 1/2 (CtBP1/2) as components of the PRDM14-mediated repressive complex. CtBP1/2 binding to PRDM14 depends on CBFA2T2, a core component of the PRDM14 complex. The loss of Ctbp1/2 impaired the PRDM14-mediated transcriptional repression required for pluripotency maintenance and primed to naïve pluripotency transition. Furthermore, CtBP1/2 also interacted with the PRC2 complexes, and the loss of Ctbp1/2 impaired the PRC2 and H3K27me3 enrichment at the target genes upon PRDM14 overexpression. These results suggest that evidence that the target gene-dependent transcriptional activity of PRDM14 is regulated by partner switching to ensure transition from primed to naïve pluripotency.
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| Overall design |
We anlalyzed 8 samples on two strip of microarray detected by GeneAtlas.
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| Contributor(s) |
Seki Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jul 22, 2019 |
| Last update date |
Jul 17, 2020 |
| Contact name |
Yoshiyuki Seki |
| E-mail(s) |
yseki@kwansei.ac.jp
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| Organization name |
Kwansei Gakuin University
|
| Department |
School of Biological and Environmental Sciences
|
| Street address |
1, Gakuen Uegahara
|
| City |
Sanda |
| State/province |
hyogo |
| ZIP/Postal code |
6691330 |
| Country |
Japan |
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| Platforms (1) |
| GPL17400 |
[MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript (gene) version] |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA556017 |
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