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| Status |
Public on Apr 28, 2004 |
| Title |
Pre-analytical changes of mRNA Gene Expression as the result from prolonged storage |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Impact of pre-analytical handling on Bone Marrow (BM) mRNA Gene Expression: We have investigated the impact of RNA extraction protocols and time delays (24 and 48h) between sample aspiration and RNA extraction on RNA quality and gene expression profiles. Intact RNA can be extracted from BM samples stored at room temperature for up to 48h after aspiration. However, storage of BM has dramatic effects on mRNA expression of individual transcripts.
Keywords: time-course
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| Contributor(s) |
Nees M, Breit S |
| Citation(s) |
15238145 |
| Submission date |
Apr 26, 2004 |
| Last update date |
Dec 13, 2018 |
| Contact name |
Matthias Nees |
| E-mail(s) |
matthias.nees@med.uni-heidelberg.de
|
| Phone |
++49-6221-564583
|
| Fax |
+49-6221-564580
|
| URL |
http://www.med.uni-heidelberg.de/kinder/onko-haemo-immuno.html
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| Organization name |
Heidelberg University, Children's Hospital
|
| Department |
Pediatric Oncology & Hematology
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| Lab |
Molecular Genetics
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| Street address |
Im Neuenheimer Feld 156
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| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
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| Platforms (1) |
| GPL8300 |
[HG_U95Av2] Affymetrix Human Genome U95 Version 2 Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA90095 |
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