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Status |
Public on Jun 30, 2022 |
Title |
miRNA expression profiling in cervical cancer cells following curcumin treatment |
Organism |
Homo sapiens |
Experiment type |
Non-coding RNA profiling by array
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Summary |
Cervical cancer is the second most common cancer in women worldwide. In addition to the important role played by HPV, the underlying mechanism promoting cervical tumorigenesis is complex and involves deregulation of key signaling pathways. Recently, role of miRNA mediated abnormal regulatory mechanisms is implicated in the pathogenesis of cervical cancer. Micro RNAs are regulatory, non‐coding RNAs about 21–23 nucleotides in length and effects the expression of a number of genes at the post‐transcriptional level. For the past few decades, role of curcumin in inhibiting the growth of cervical cancer and increasing the chemo and radio- sensistivity has been studied extensively. Interestingly, curcumin was shown to downregulate NF-κB, Wnt/β-catenin, TGF‐β and various other signaling pathways in cervical cancer cells. Although, a number of microarray studies have examined the use of miRNAs as cancer diagnostic markers, the regulation of miRNAs upon treatment with curcumin in cervical cancer cells has not been studied. The current study is aimed to perform miRNA profiling in cervical cancer cells following curcumin treatment and to study the role of miRNAs in regulating the different signaling pathways.
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Overall design |
miRNA profiling was performed at 24 h following curcumin treatment (n=3) to SiHa cervical cancer cells. DMSO (0.4%) was used as a vehicle control (n=3) for all the experiments. Agilent one-color experiment,Organism: Homo sapiens ,Agilent Human miRNA 8x60k Arrays AMADID: 070156
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Contributor(s) |
Samji P, Ganesh A, Warrier VP, Karunagaran MK |
Citation missing |
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Submission date |
Jul 23, 2019 |
Last update date |
Jul 02, 2022 |
Contact name |
Genotypic technology |
E-mail(s) |
sudha.rao@genotypic.co.in
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Organization name |
Genotypic Technology
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Street address |
259, Apoorva 4th cross,80 feet Road,RMV 2ND STAGE
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City |
Bangalore |
State/province |
Karnataka |
ZIP/Postal code |
560094 |
Country |
India |
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Platforms (1) |
GPL25134 |
Agilent-070156 Human_miRNA_V21.0_Microarray 046064 (miRNA ID version) |
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Samples (6)
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Relations |
BioProject |
PRJNA556228 |
Supplementary file |
Size |
Download |
File type/resource |
GSE134723_RAW.tar |
41.3 Mb |
(http)(custom) |
TAR (of TXT) |
Processed data included within Sample table |
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