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Status |
Public on Dec 09, 2020 |
Title |
ChIP assay in adult ventricular cardiomyocytes |
Organism |
Rattus norvegicus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
ChIP assay was performed to study the genomic location of SRF myocytes cistrome in adult ventricular cardiomyocytes. The myocytes were stimulated by phenylephrine (PE) combined with adenoviral overexpression of SRF serine-103 mutants.
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Overall design |
Adult ventricular cardiomyocytes were stimulated with PE 24 hours after adenoviral infection with SRF WT, S103A and S103D mutants. Cells were fixed 1 hour after PE treatment, then subjected to Chromatin Immunoprecipitation (ChIP) by PolII, H3K27ac, Flag, SRF and p-SRF(s103) antibodies.
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Contributor(s) |
Li J, Tan Y |
Citation(s) |
32933333 |
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Submission date |
Jul 24, 2019 |
Last update date |
Dec 09, 2020 |
Contact name |
Yuliang Tan |
E-mail(s) |
yut020@ucsd.edu
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Organization name |
University of California, San Diego
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Department |
School of Medicine
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Street address |
Room 345, CMM West, 9500 Gilman Drive, Mail Code 0648
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City |
La Jolla |
State/province |
California |
ZIP/Postal code |
92037-0648 |
Country |
USA |
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Platforms (1) |
GPL22396 |
Illumina HiSeq 4000 (Rattus norvegicus) |
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Samples (60)
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This SubSeries is part of SuperSeries: |
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Relations |
BioProject |
PRJNA556435 |
SRA |
SRP216248 |