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Series GSE134799 Query DataSets for GSE134799
Status Public on Dec 09, 2020
Title ChIP assay in adult ventricular cardiomyocytes
Organism Rattus norvegicus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary ChIP assay was performed to study the genomic location of SRF myocytes cistrome in adult ventricular cardiomyocytes. The myocytes were stimulated by phenylephrine (PE) combined with adenoviral overexpression of SRF serine-103 mutants.
 
Overall design Adult ventricular cardiomyocytes were stimulated with PE 24 hours after adenoviral infection with SRF WT, S103A and S103D mutants. Cells were fixed 1 hour after PE treatment, then subjected to Chromatin Immunoprecipitation (ChIP) by PolII, H3K27ac, Flag, SRF and p-SRF(s103) antibodies.
 
Contributor(s) Li J, Tan Y
Citation(s) 32933333
Submission date Jul 24, 2019
Last update date Dec 09, 2020
Contact name Yuliang Tan
E-mail(s) yut020@ucsd.edu
Organization name University of California, San Diego
Department School of Medicine
Street address Room 345, CMM West, 9500 Gilman Drive, Mail Code 0648
City La Jolla
State/province California
ZIP/Postal code 92037-0648
Country USA
 
Platforms (1)
GPL22396 Illumina HiSeq 4000 (Rattus norvegicus)
Samples (60)
GSM3970428 Input_11
GSM3970429 Input_12
GSM3970430 Input_13
This SubSeries is part of SuperSeries:
GSE134801 Adult ventricular cardiomyocytes
Relations
BioProject PRJNA556435
SRA SRP216248

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE134799_RAW.tar 9.9 Gb (http)(custom) TAR (of BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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