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Series GSE134977 Query DataSets for GSE134977
Status Public on Jul 27, 2019
Title Next Generation Sequencing Quantitative Analysis of Small RNA in Livers of Adult Male Offspring of Obese Female Mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Obesity during pregnancy is associated with increased risk of non-alcoholic fatty liver disease in the offspring. We used RNA next generation sequencing analysis (HiSeq2000) to have a glance at the miRNome in twelve week-old male offspring exposed to maternal obesity and weaned onto an obesogenic diet.
 
Overall design Thirty-five female C57BL/6J mice were fed either a standard control diet, RM1 [7% simple carbohydrates/3% lipids (w/w)] or an energy-rich obesogenic diet [10% simple carbohydrates/20% lipids (w/w)] plus sweetened condensed milk [55% simple carbohydrates, 8% lipids, 8% protein (w/w), supplemented with mineral and vitamin mix AIN93G]. Female mice were mated, and maintained on their diets until weaning. On post-natal day 21, male offspring were weaned onto either standard chow (RM1) or the obesogenic diet, generating 4 experimental groups: maternal and offspring control diet (CC), maternal control diet and offspring obesogenic diet (CO), maternal obesogenic diet and offspring control diet (OC), maternal and offspring obesogenic diet (OO). At 12 weeks of age, mice were killed by raising on a fasted state. Liver were dissected, and snap frozen. Three high quality RNA samples from each group were randomly selected and subjected to a cDNA library preparation for further sRNA sequencing using TruSeq RNA Library Preparation kit
 
Contributor(s) Pantaleao LC, Ozanne SE
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Submission date Jul 26, 2019
Last update date Jul 28, 2019
Contact name Lucas Pantaleao
E-mail(s) lp435@medschl.cam.ac.uk
Phone +447842771748
Organization name University of Cambridge
Street address 11A Glisson Road
City Cambridge
ZIP/Postal code CB1 2HA
Country United Kingdom
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (12)
GSM3983033 RC105A [miRNA]
GSM3983034 RC83A [miRNA]
GSM3983035 RC82A [miRNA]
Relations
BioProject PRJNA556904
SRA SRP216545

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Supplementary file Size Download File type/resource
GSE134977_mature_sense_1_4.mat.txt.gz 40.5 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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