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| Status |
Public on Aug 01, 2019 |
| Title |
RNA Sequencing Facilitates Quantitative Analysis of Transcriptomes of H1 derived cells and H1 after MSX2-knockout derived cells at Day8 after human early hematopoietic differentiation . |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: The goals of this study are to verify MSX2 knock is suffice to promote hematopoiesis during human early hematopoietic differentiation through comparing the transcriptome profilings in WT samples and MSX2-knockout samples collected at day 8 after hematopoietic differentiation.
Method: mRNA profiles of hESC samples collected at day 8 after hematopoiesis differentiation were generated by deep sequencing using Illumina GAIIx. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using TaqMan and SYBR Green assays
Conclusions: MSX2 knock is suffice to promote hematopoiesis during early hematopoietic differentiation of H1 cells.
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| Overall design |
mRNA profiles of hESC samples collected at day 8 after hematopoiesis differentiation were generated by deep sequencing using Illumina GAIIx. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks.
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| Contributor(s) |
Zhou J, Wang H |
| Citation(s) |
32248833 |
| |
| Submission date |
Jul 31, 2019 |
| Last update date |
Apr 20, 2020 |
| Contact name |
Jiaxi Zhou |
| E-mail(s) |
leisheng_zhang@163.com
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| Phone |
+86-22-23909101
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| Organization name |
PUMC
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| Street address |
288# Nanjing Road, He ping district
|
| City |
Tianjin |
| State/province |
Tianjin |
| ZIP/Postal code |
300020 |
| Country |
China |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA557665 |
| SRA |
SRP216905 |
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