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| Status |
Public on Aug 05, 2021 |
| Title |
TonEBP-deficiency accelerates age-dependent intervertebral disc degeneration underscored by matrix remodeling, cytoskeletal rearrangements, and changes in proinflammatory gene expression |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Our studies show that TonEBP-deficiency causes pronounced degeneration of all three intervertebral disc compartments with greater incidence of herniation in the mouse. The disc phenotype is marked by extracellular matrix remodeling, actin cytoskeleton rearrangements, and suppressed proinflammatory gene expression, advancing our understanding of the contributions of TonEBP in intervertebral disc homeostasis and disease.
We used microarray to explore the transcriptomics of differentially expressed genes of annulus fibrosus (AF) and nucleus pulposus (NP) tissue in TonEBP haploinsufficient mice on a C57BL/6 background.
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| Overall design |
AF and NP tissue were dissected from lumbar discs (L1-S1) of 12-month-old TonEBP haploinsufficient and wild-type mice (n=3). RNA extraction and hybridization on Affymetrix microarrays.
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| Contributor(s) |
Tessier S, Novais EJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 01, 2019 |
| Last update date |
Aug 06, 2021 |
| Contact name |
Steven Tessier |
| E-mail(s) |
tue43401@temple.edu
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| Phone |
717-575-6788
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| Organization name |
Thomas Jefferson
|
| Department |
Orthopedic Surgery
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| Lab |
Makarand V. Risbud
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| Street address |
3700 Gateway Dr., Apt 622
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| City |
Philadelphia |
| State/province |
Pennsylvania |
| ZIP/Postal code |
19145 |
| Country |
USA |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA558075 |
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