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| Status |
Public on Apr 19, 2021 |
| Title |
Activation of ERRĪ³ enhances mitochondrial and sarcomere maturation in human induced pluripotent stem cell-derived cardiomyocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We identified two cardiac maturation stimulators from 9,048 compounds using Troponin I1 (TNNI1) and Troponin I3 (TNNI3) double reporter hiPSC-derived CMs. An estrogen-related receptor gamma (ERRĪ³)-specific agonist increased the size of hiPSC-CMs and their mitochondrial content and enhanced their mitochondrial function. In addition, combination of this agonist with an inhibitor of S-phase kinase-associated protein 2 (SKP2) increased the TNNI3 expression more highly than previous methods and enhanced cardiac maturation-related gene expression profiles.
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| Overall design |
mRNA profiles of hiPSC-CMs with conpounds were generated by deep sequencing, in triplicate, using Illumina Nextseq 500.
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| Contributor(s) |
Miki K, Monjo T, Andou T, Sasaki M, Lucena-Cacace A, Nishimoto T, Yoshida Y |
| Citation(s) |
34155205 |
| |
| Submission date |
Aug 02, 2019 |
| Last update date |
Jul 14, 2021 |
| Contact name |
Yoshinori Yoshida |
| E-mail(s) |
yoshinor@cira.kyoto-u.ac.jp
|
| Organization name |
Kyoto University
|
| Street address |
53, Kawahara-cho, Shogoin, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
6068507 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA558440 |
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