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Series GSE13543 Query DataSets for GSE13543
Status Public on May 01, 2009
Title B. anthracis transcriptome analysis using Illumina and SOLiD sequencing
Organism Bacillus anthracis
Experiment type Expression profiling by high throughput sequencing
Summary Although gene expression has been studied in bacteria for decades, many aspects of the bacterial transcriptome remain poorly understood. Transcript structure, operon linkages, and absolute abundance information all provide valuable insights into gene function and regulation, but none has ever been determined on a genome-wide scale for any bacterium. Here we report the first comprehensive and unbiased definition of a bacterial transcriptome under various growth conditions. Using a high throughput sequencing approach, we mapped transcript start sites and operons throughout the genome, and enhanced existing genome annotations. Further, our data provide estimates of absolute transcript abundance, and suggest there is significant transcriptional heterogeneity within a clonal, synchronized bacterial population. Overall, our results offer an unprecedented view of gene expression and regulation in a bacterial cell.
Overall design Includes:

Comparison of transcriptomes under 2 different atmospheric conditions using Illumina sequencing (2 Samples)


Comparison of transcriptomes in different growth phases under 2 different atmospheric conditions using SOLiD sequencing (6 Samples).
Contributor(s) Passalacqua KD, AV, Ondov BD, Okou DT, Zwick ME, Bergman NH
Citation(s) 19304856
Submission date Nov 10, 2008
Last update date May 15, 2019
Contact name Nicholas Bergman
Phone 404 894 8418
Organization name Georgia Institute of Technology
Department Biology
Street address 310 Ferst Dr., Rm 231
City Atlanta
State/province GA
ZIP/Postal code 30332
Country USA
Platforms (2)
GPL9410 Illumina Genome Analyzer (Bacillus anthracis)
GPL9411 AB SOLiD System (Bacillus anthracis)
Samples (8)
GSM341367 Mid-log_air_rep1_Illumina
GSM341368 Mid-log_CO2_rep1_Illumina
GSM343458 Early-log_air_SOLiD
SRA SRP001274
BioProject PRJNA110721

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE13543_RAW.tar 13.1 Gb (http)(custom) TAR (of QUAL, TXT)
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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