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| Status |
Public on Feb 24, 2021 |
| Title |
RNF40 exerts stage-dependent functions in differentiating osteoblasts and is essential for bone cell crosstalk [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In this study, we focused on elucidating the role of Ring Finger Protein 40 (RNF40) in bone development by using a conditional knockout mouse approach during different stages of osteoblast (OB) differentiation and maturation. RNF40 forms an obligate E3 ubiquitin ligase complex together with RNF20 and mediates the monoubiquitination of lysine 120 of histone H2B (H2BK120ub1). We provide evidence that RNF40 regulates OB differentiation in a stage-dependent manner. Additionally, we show that RNF40 is required for bone cell crosstalk whereby loss of RNF40 leads to a reduction in osteoclast numbers and function through modulation of vitamin D receptor (VDR)-induced Rankl expression in OBs. Taken together, these data imply an important role of RNF40-mediated H2Bub1 in bone formation and remodeling and provide a basis for further investigation of its anti-resorptive potential for the treatment of conditions such as osteoporosis or cancer-associated osteolysis.
In this study we show that RNF40 is required in earlier stages of osteoblast differentiation and is involved in bone cell crosstalk by regulating vitamin D induced Rankl expression
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| Overall design |
We generated RNA-Sequencing data from calvarial osteoblasts isolated from RNF40RosaCreERT2 mutant mice.
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| Contributor(s) |
Johnsen SA, Najafova Z |
| Citation(s) |
32901120 |
| Submission date |
Aug 09, 2019 |
| Last update date |
Feb 24, 2021 |
| Contact name |
Steven A. Johnsen |
| Organization name |
Robert Bosch Center for Tumor Diseases
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| Lab |
Molecular Cancer Epigenetics
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| Street address |
Auerbachstraße 112
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| City |
Stuttgart |
| State/province |
BW |
| ZIP/Postal code |
70376 |
| Country |
Germany |
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| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE135629 |
RNF40 exerts stage-dependent functions in differentiating osteoblasts and is essential for bone cell crosstalk |
|
| Relations |
| BioProject |
PRJNA559511 |
| SRA |
SRP217927 |
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