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| Status |
Public on Jan 24, 2020 |
| Title |
Single-Cell Transcriptomics of Genome-Edited Kidney Organoids |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report the first use of genome-edited human kidney organoids, combined with single-cell transcriptomics, to study APOL1 risk variants at the native genomic locus in different nephron cell types. This approach captures interferon-mediated induction of APOL1 gene expression and cellular dedifferentiation with a secondary insult“second hit” of endoplasmic reticulum stress.
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| |
|
| Overall design |
Examination of interferon gamma +/- tunicamycin in G0 and G1 APOL1 kidney organoids. A, B, C, etc refer to independent experimental replicates.
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| |
|
| Contributor(s) |
Lin J, Radmanesh B |
| Citation(s) |
32656538 |
| |
| Submission date |
Aug 09, 2019 |
| Last update date |
Jul 15, 2020 |
| Contact name |
Behram Sarosh Radmanesh |
| E-mail(s) |
behram.radmanesh@northwestern.edu
|
| Organization name |
Northwestern University
|
| Street address |
633 Clark St
|
| City |
Evanston |
| State/province |
IL |
| ZIP/Postal code |
60208 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (16)
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| Relations |
| BioProject |
PRJNA559570 |
| SRA |
SRP217972 |
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