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| Status |
Public on Mar 30, 2009 |
| Title |
US28-expressing and mock-transfected stable NIH-3T3 cell lines |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The human cytomegalovirus (HCMV) encodes the chemokine receptor US28 that exhibits constitutive activity. NIH-3T3 cells stably transfected with US28 present a pro-angiogenic and transformed phenotype both in vitro and in vivo.
We used gene expression profiling to determine how US28 constitutive activity modulates expression of genes compared to mock-transfected cells.
Keywords: Comparison of stably transfected cell lines
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| Overall design |
We isolated RNA from two different clonal stable cell lines of NIH-3T3 cells transfected either either mock or US28-WT and analysed these 4 RNA samples using Affymetrix mouse genome arrays.
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| Contributor(s) |
Maussang D, Langemeijer E, Fitzsimons CP, Stigter-van Walsum M, Dijkman R, Borg MK, Slinger E, Schreiber A, Michel D, Tensen CP, van Dongen GA, Leurs R, Smit MJ |
| Citation(s) |
19318580 |
| |
| Submission date |
Nov 12, 2008 |
| Last update date |
Feb 11, 2019 |
| Contact name |
David Maussang |
| E-mail(s) |
dmaussa@few.vu.nl
|
| Organization name |
Vrije Universiteit Amsterdam, Leiden/Amsterdam Center for Drug Research
|
| Department |
Division of Medicinal Chemistry, Faculty of Sciences
|
| Lab |
Martine J Smit/Rob Leurs
|
| Street address |
De Boelelaan 1083
|
| City |
Amsterdam |
| ZIP/Postal code |
1081 HV |
| Country |
Netherlands |
| |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA110669 |
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