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Series GSE136132 Query DataSets for GSE136132
Status Public on Aug 21, 2023
Title Expression data from primary murine CD8+ T cells incubated in AML cell or dendritic cell conditioned medium
Organism Mus musculus
Experiment type Expression profiling by array
Summary During activation T cells need to undergo metabolic reprogramming for proper effector function. Disturbances in metabolic pathways lead to impaired T cell function. Active metabolic evasion of cancer cells lead to less immunosurveillance and uncontrolled tumor growth.
We used microarrays to investigate the expression changes in T cells challenged with AML supernatants shedding light on the underlying mechanisms of metabolic evasion in leukemia.
 
Overall design Primary murine T cells isolated from the spleen were activated and cultured in fresh medium (Tc.Tc) as a control, medium conditioned by WEHI 3B murine AML cells (Tc.WEHI) or bone marrow derived dendritic cells (Tc.Dc). CD8+ T cells were reisolated after 48 hrs for RNA extraction and hybridization on Affymetrix microarrays. Seven biological replicates per condition were analyzed.
 
Contributor(s) Uhl F, Zeiser R, Andrieux G, Boerries M
Citation(s) 33115954
Submission date Aug 21, 2019
Last update date Aug 22, 2023
Contact name Geoffroy Andrieux
Organization name University clinics Freiburg
Street address Breisacherstr 153
City Freiburg
ZIP/Postal code 79110
Country Germany
 
Platforms (1)
GPL23038 [Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay)
Samples (21)
GSM4041499 Tc+medium[DC] biological replicate 1
GSM4041500 Tc+medium[DC] biological replicate 2
GSM4041501 Tc+medium[DC] biological replicate 3
Relations
BioProject PRJNA561354

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE136132_RAW.tar 22.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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