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| Status |
Public on Jan 01, 2020 |
| Title |
Genome-wide distribution of proteins ZAF1 and dCTCF in Drosophila embryos |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Recently, the concept has arisen that a special class of architectural proteins exists, which are responsible not only for global chromosome architecture but also for the local regulation of enhancer–promoter interactions. Here, we describe a new architectural protein, with a total size of only 375 aa, which contains an N-terminal zinc finger-associated domain (ZAD) and a cluster of five C2H2 domains at the C-terminus. This new protein, named ZAD and Architectural Function 1 protein (ZAF1 protein), is weakly and ubiquitously expressed, with the highest expression levels observed in oocytes and embryos. The cluster of C2H2 domains recognizes a specific 15-bp consensus site, located predominantly in promotors, near transcription start sites. The expression of ZAF1 by a tissue-specific promoter led to the complete blocking of the eye enhancer when clusters of ZAF1 binding sites flanked the eye enhancer in transgenic lines, suggesting that the loop formed by the ZAF1 protein leads to insulation. The ZAF1 protein also supported long-range interactions between the yeast GAL4 activator and the white promoter in transgenic Drosophila lines. A mutant protein lacking the ZAD failed to block the eye enhancer or to support distance interactions in transgenic lines. Taken together, these results suggest that ZAF1 is a minimal architectural protein that can be used to create a convenient model for studying the mechanisms of distance interactions.
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| Overall design |
ChIP-seq signal of the ZAF1 (two replicates) and dCTCF (one replicate) architectural/insulator proteins occupancy in whole embryo during embryonic development at 0-12h after egg-laying.
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| Contributor(s) |
Maksimenko O, Klimenko N |
| Citation(s) |
31706027 |
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| Submission date |
Aug 27, 2019 |
| Last update date |
Oct 13, 2020 |
| Contact name |
Natalia Klimenko |
| E-mail(s) |
lklimenko@genebiology.ru
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| Phone |
9150884603
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| Organization name |
Institute of Gene Biology (IGB) of the Russian Academy of Sciences
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| Street address |
34/5 Vavilova Street
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| City |
Moscow |
| ZIP/Postal code |
143026 |
| Country |
Russia |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA562508 |
| SRA |
SRP219901 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE136407_ZAF1_merged_vs_preimmune.BedGraph.gz |
92.9 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE136407_ZAF1_merged_vs_preimmune.narrowPeak.gz |
5.4 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE136407_dCTCF_vs_preimmune.BedGraph.gz |
28.9 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE136407_dCTCF_vs_preimmune.narrowPeak.gz |
20.7 Kb |
(ftp)(http) |
NARROWPEAK |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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