|
| Status |
Public on May 18, 2011 |
| Title |
Global effect of JTV or JTV-Alt overexpression in HeLa cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
JTV is not only a structural component of multi-aminoacyl-tRNA synthetase complex, but also a regulator of FBP that in turn controls c-Myc expression. In order to identify JTV target genes other than FBP and c-Myc, JTV or JTV-Alt (alternatively spliced form of JTV, enriched in the nucleus) protein was over-expressed in HeLa cells and global gene expression changes were monitored using cDNA expression microarray.
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| |
|
| Overall design |
JTV or JTV-Alt was expressed in HeLa cells by transient transfection as protein fusion with GFP. After harvesting, only GFP-positive cells were isolated by flow cytometry, and used for RNA extraction. Each JTV or JTV-Alt sample was analyzed by co-hybridizing with control GFP-only samples. Each of the two forms of JTV hybridization was repeated as biological replicate.
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| |
|
| Citation(s) |
21285945 |
| |
| Submission date |
Nov 18, 2008 |
| Last update date |
Mar 20, 2012 |
| Contact name |
David Levens |
| E-mail(s) |
levens@helix.nih.gov
|
| Phone |
301-496-2176
|
| Fax |
301-402-0043
|
| Organization name |
NIH
|
| Department |
NCI/CCR
|
| Lab |
LP
|
| Street address |
10 Center Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
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| Platforms (1) |
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| Samples (4)
|
| GSM343494 |
GFP-JTV Transfection - repeat 1 - mAdbID:75970 |
| GSM343495 |
GFP-JTV-Alt Transfection - repeat 1 - mAdbID:75971 |
| GSM343496 |
GFP-JTV Transfection - repeat 2 - mAdbID:77132 |
| GSM343497 |
GFP-JTV-Alt Transfection - repeat 2 - mAdbID:77133 |
|
| Relations |
| BioProject |
PRJNA110197 |
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