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Series GSE13654 Query DataSets for GSE13654
Status Public on May 12, 2009
Title Affinity purification of ribosomes and associated RNAs using tagged Rpl16a and Rpl16b
Organism Saccharomyces cerevisiae
Experiment type Other
Summary In this study, we systematically identified RNAs associated with ribosomes. To identify ribosome associated RNAs, C-terminal ZZ-tagged Rpl16a or Rpl16b, expressed under control of thier native promoter, were affinity purified from whole cell extracts of cultures grown to mid-log phase in minimal medium. Extracts were incubated with immunoglobulin G (IgG) coupled microbeads, washed, and ribosomes were eluted by tobacco etch virus (TEV) protease treatment. We performed two biological replicates with each protein and analyzed the RNA content using oligo microarrays. Total RNA isolated from extracts of cells expressing Rpl16a-ZZ or Rpl16b-ZZ (input) and from the affinity-purified ribosomes was reverse transcribed with oligo(dT) primers. cDNA was labeled with Cy3 and Cy5 fluorescent dyes, respectively, and competitively hybridized to yeast oligo microarrays. Alternatively, RNA was isolated from sucrose gradient fractions containing 60S subunits, 80S monosomes and polysomes. Here, we performed four biological replicates. Analysis was done with oligo microarrays as described above.
A strain or line experiment design type assays differences between multiple strains, cultivars, serovars, isolates, lines from organisms of a single species.
Overall design strain_or_line_design
Contributor(s) Halbeisen R
Citation(s) 19419242
Submission date Nov 18, 2008
Last update date Mar 20, 2012
Contact name Andre Gerber
Phone +41 44 6337378
Fax +41 44 6337364
Organization name Swiss Federal Institute of Technology
Department Institute of Pharmaceutical Science
Street address Wolfgang Pauli Str. 10, HCI H396
City ETH Hoenggerberg, Zuerich
ZIP/Postal code ZH
Country Switzerland
Platforms (1)
GPL8546 SMD Print_1548 Saccharomyces cerevisiae
Samples (8)
GSM402426 Rpl16a-ZZ Gradient (2)
GSM402427 Rpl16a-ZZ in SC (1)
GSM402428 Rpl16b-ZZ in SC (2)
This SubSeries is part of SuperSeries:
GSE13682 Affinity purification of ribosomes and associated RNAs from stress-treated cells using tagged Rpl16a and Rpl16b
BioProject PRJNA113917

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE13654_RAW.tar 1.0 Mb (http)(custom) TAR
Processed data included within Sample table

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