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| Status |
Public on Sep 04, 2019 |
| Title |
The U-box family genes in Medicago truncatula: key elements in response to salt, cold, and drought stresses |
| Organism |
Medicago truncatula |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The ubiquitination pathway regulates growth, development, and stress responses in plants, and the U-box protein family of ubiquitin ligases has important roles in this pathway. Here, 64 putative U-box proteins were identified in the Medicago truncatula genome. In addition to the conserved U-box motif, other functional domains, such as the ARM, kinase, KAP, and WD40 domains, were also detected. Phylogenetic analysis of the M. truncatula U-box proteins grouped them into six subfamilies, and chromosomal mapping and synteny analyses indicated that tandem and segmental duplications may have contributed to the expansion and evolution of the U-box gene family in this species. Using RNA-seq data from M. truncatula seedlings subjected to three different abiotic stresses, we identified 33 stress-inducible plant U-box genes (MtPUBs). Specifically, 25 salinity-, 15 drought-, and 16 cold-regulated MtPUBs were detected. Among them, MtPUB10, MtPUB17, MtPUB18, MtPUB35, MtPUB42, and MtPUB44 responded to all three stress conditions. Expression profiling by real-time PCR was consistent with the RNA-seq data, and stress-related elements were identified in the promoter regions. The present findings strongly indicate that U-box proteins play critical roles in abiotic stress response in M. truncatula.
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| Overall design |
RNA-seq in Medicago truncatula
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| Contributor(s) |
Song J |
| Citation(s) |
31505820 |
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| Submission date |
Sep 03, 2019 |
| Last update date |
Oct 21, 2019 |
| Contact name |
Jianbo Song |
| E-mail(s) |
thinkskier@163.com
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| Phone |
18579059335
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| Organization name |
Jiang Xi Agricultural University
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| Street address |
Department of Biochemistry and Molecular Biology, College of Science, Jiang Xi Agricultural University, Nanchang 330045, China
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| City |
Nanchang |
| State/province |
Jiang Xi |
| ZIP/Postal code |
330045 |
| Country |
China |
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| Platforms (1) |
| GPL23002 |
Illumina HiSeq 3000 (Medicago truncatula) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA563553 |
| SRA |
SRP220100 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE136739_RAW.tar |
4.7 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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