Genome binding/occupancy profiling by high throughput sequencing
Summary
CD34+ Haematopoietic stem cells were differentiated under two ex vivo protocols to generate ATAC-seq data for machine learning of rules underlying open chromatin dynamics.
Overall design
CD34+ cells were isolated from peripheral blood from three healthy donors (Two males, one female) and differentiatied ex vivo using two different protocols. The first protocol (P1) uses a 2-phase differentiation and the second protcol (P2) uses a 3-phase differentiation which includes co-culture on a layer of stromal MS-5 cells. ATAC-seq was perfroemd at various days throughout differentiation.
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