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| Status |
Public on Jun 28, 2021 |
| Title |
Identification of miRNAs as biomarkers of drug response of Pien Tze Huang in Hepatic Fibrosis Treatments |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Pien Tze Huang (PZH) is herbal traditional Chinese medicine which was widely utilized in Asia for hepatic diseases. We constructed two groups hepatic fibrosis mice model using CCl4, one group using PZH treatment and another group replacing PZH with double distilled water. All 12 mice were fed for 8 weeks and then killed to have their liver tissue taken out. Small RNA-seq were used to identify miRNAs of PZH medicine effect for hepatic fibrosis. We found the expression of these miRNAs (mmu-miR-205-5p, mmu-miR-3064-5p, mmu-miR-205-5p, mmu-miR-370-3p, mmu-miR-665-3p) were changed in PZH medicine treatment for hepatic fibrosis study. Furthermore, Hmga2 and Fgf9, miRNAs corresponding target genes (Sp4, Slc2a6, Tln2, Hmga2, Ank3, Pax9, Fgf9), have been reported association with hepatic fibrosis.
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| Overall design |
We constructed two groups hepatic fibrosis mice model using CCl4, one group using PZH treatment and another group replacing PZH with double distilled water. Small RNA-seq were used to identify miRNAs of PZH medicine effect for hepatic fibrosis.
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| Contributor(s) |
Qin S, Zhang D, Zhu J |
| Citation missing |
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| Submission date |
Sep 09, 2019 |
| Last update date |
Jun 28, 2021 |
| Contact name |
Jinhang Zhu |
| E-mail(s) |
zhujinhang@yeah.net
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| Phone |
15855169341
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| Organization name |
Shanghai Jiao Tong University
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| Street address |
Huashan Road 1954
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| City |
Shanghai |
| ZIP/Postal code |
200030 |
| Country |
China |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA564553 |
| SRA |
SRP221041 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE137100_12_sample_1929_miRNA_rawExpmatrix.txt.gz |
30.8 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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