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| Status |
Public on May 23, 2009 |
| Title |
Differential gene expression of young and senescent HUVECs under static and laminar shear stress conditions |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Laminar shear stress due to constant blood flow is known to play a critical role in maintaining vascular health. In contrast, endothelial cell senescence appears to be closely associated with the incidence of vascular disorder. In an attempt to identify functional biomarkers for age-related vascular health/disease, the present study investigated differential gene expression of young and senescent human umbilical vein endothelial cells (HUVECs) under static and laminar shear stress.
We used a cDNA microarray method to compare gene expression profiles of young and senescent HUVECs under static and laminar shear stress conditions.
Keywords: stress response, age state analysis
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| Overall design |
Senescent cells were prepared by continuous subculture in vitro, and a cone-and-plate device was used to impose laminar shear stress onto cells. Young and senescent cells were exposed to laminar shear stress or maintained under static conditions. Total mRNA was extracted and gene expression profiles were analyzed by cDNA microarray.
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| |
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| Contributor(s) |
Mun GI, Boo YC |
| Citation(s) |
19409979, 20382854 |
| |
| Submission date |
Nov 23, 2008 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Yong Chool Boo |
| E-mail(s) |
ycboo@knu.ac.kr
|
| Organization name |
Kyungpook National University School of Medicine
|
| Department |
Department of Molecular Medicine
|
| Lab |
Free Radical Biology
|
| Street address |
101 Dongindong
|
| City |
Daegu |
| ZIP/Postal code |
700-422 |
| Country |
South Korea |
| |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (12)
|
| GSM344778 |
Vein endothelial cells_Young_Static_rep1 |
| GSM344779 |
Vein endothelial cells_Young_Static_rep2 |
| GSM344780 |
Vein endothelial cells_Young_Static_rep3 |
| GSM344781 |
Vein endothelial cells_Young_Shear_rep1 |
| GSM344782 |
Vein endothelial cells_Young_Shear_rep2 |
| GSM344783 |
Vein endothelial cells_Young_Shear_rep3 |
| GSM344784 |
Vein endothelial cells_Old_Static_rep1 |
| GSM344785 |
Vein endothelial cells_Old_Static_rep2 |
| GSM344786 |
Vein endothelial cells_Old_Static_rep3 |
| GSM344787 |
Vein endothelial cells_Old_Shear_rep1 |
| GSM344788 |
Vein endothelial cells_Old_Shear_rep2 |
| GSM344789 |
Vein endothelial cells_Old_Shear_rep3 |
|
| Relations |
| BioProject |
PRJNA110385 |
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