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| Status |
Public on Jul 29, 2020 |
| Title |
Long noncoding RNA INCA1 regulates tumor interferon signaling |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Tumor interferon signaling regulates the expression of immunosuppressive molecules and promotes cancer immune evasion. Although the role of long noncoding RNAs (lncRNAs) in the regulation of gene expression is now emerging, their function in tumor interferon signaling remains unexplored. We have identified the interferon-γ (IFNγ)-stimulated non-coding RNA 1 (INCA1) as a novel lncRNA expressed form the PD-L1 locus. INCA1 is expressed in multiple tumor types and its levels increase after IFNγ stimulation. In this study we performed transcriptome analysis (RNA-seq) of INCA1 knockdown cells and show that INCA1 regulates the expression of several interferon-stimulated genes. Overall, our findings reveal INCA1 as a critical component of the tumor interferon signaling.
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| Overall design |
Tumor cell lines stable expressing shRNA control or shRNA targeting INCA1 were stimulated with interferon gamma for 24 hours. RNA expression profiles were generated by RNA-seq, in triplicate, using Illumina NovaSeq6000.
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| Contributor(s) |
Mineo M, Chiocca EA |
| Citation(s) |
32504554 |
| Submission date |
Sep 15, 2019 |
| Last update date |
Jul 29, 2020 |
| Contact name |
Marco Mineo |
| E-mail(s) |
mmineo@bwh.harvard.edu
|
| Phone |
6175255656
|
| Organization name |
Brigham and Women's Hospital
|
| Street address |
60 Fenwood Rd, BTM 8012
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE137489 |
Long noncoding RNA INCA1 regulates tumor interferon signaling. |
|
| Relations |
| BioProject |
PRJNA565585 |
| SRA |
SRP221667 |
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