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Series GSE137473 Query DataSets for GSE137473
Status Public on Jun 10, 2020
Title A systems approach delivers a functional microRNA catalog and expanded targets for seizure suppression in temporal lobe epilepsy
Organisms Mus musculus; Rattus norvegicus
Experiment type Non-coding RNA profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary MicroRNAs (miRNAs) are short noncoding RNAs that shape the gene expression landscape, including during the pathogenesis of temporal lobe epilepsy (TLE). In order to provide a full catalog of the miRNA changes that happen during experimental TLE, we sequenced Argonaute 2-loaded miRNAs in the hippocampus of three different animal models at regular intervals between the time of the initial precipitating insult to the establishment of spontaneous recurrent seizures. The commonly upregulated miRNAs were selected for a functional in vivo screen using oligonucleotide inhibitors. This revealed anti-seizure phenotypes upon inhibition of miR-10a-5p, miR-21-5p and miR-142-5p as well as neuroprotection-only effects for inhibition of miR-27a-3p and miR-431-5p. Pathway enrichment analysis on predicted and validated targets of these miRNAs indicated a role for TGFβ signaling in a shared seizure-modifying mechanism. Together, these results identify functional miRNAs in the hippocampus and a pipeline of new targets for seizure control in epilepsy.
 
Overall design small RNA sequencing of Ago2-loaded miRNAs from three different rodent models across all phases of epilepsy development, two in mouse one in rat. Each model is sequenced in timepoints during epileptogenesis and at chronic phase. Each group is in triplicate.
 
Contributor(s) Veno MT, Kjems J, Henshall DC
Citation(s) 32581127, 33584828
Submission date Sep 16, 2019
Last update date Feb 16, 2021
Contact name Morten T Veno
E-mail(s) morten.veno@omiics.com
Phone +45 28727107
Organization name Omiics ApS
Street address Aabogade 15
City Aarhus N
State/province DK
ZIP/Postal code 8200
Country Denmark
 
Platforms (3)
GPL19057 Illumina NextSeq 500 (Mus musculus)
GPL20084 Illumina NextSeq 500 (Rattus norvegicus)
GPL22396 Illumina HiSeq 4000 (Rattus norvegicus)
Samples (114)
GSM4079910 MRP1_RH_24: Rattus norvegicus, perforant pathway stimulation (PPS), 1 hour
GSM4079911 MRP1_RH_26: Rattus norvegicus, perforant pathway stimulation (PPS), 1 hour
GSM4079912 MRP1_RH_36: Rattus norvegicus, perforant pathway stimulation (PPS), 1 hour
Relations
BioProject PRJNA565693
SRA SRP221723

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE137473_IAKA_miRNA_raw_count.txt.gz 53.5 Kb (ftp)(http) TXT
GSE137473_PILO_miRNA_raw_count.txt.gz 57.2 Kb (ftp)(http) TXT
GSE137473_PPS_miRNA_raw_count.txt.gz 24.8 Kb (ftp)(http) TXT
GSE137473_gene_exp.diff_MRP2.vs.MRP8.txt.gz 1.6 Mb (ftp)(http) TXT
GSE137473_gene_exp.diff_MRP3.vs.MRP8.txt.gz 1.6 Mb (ftp)(http) TXT
GSE137473_gene_exp.diff_MRP4.vs.MRP8.txt.gz 1.6 Mb (ftp)(http) TXT
GSE137473_gene_exp.diff_MRP6.vs.MRP8.txt.gz 1.6 Mb (ftp)(http) TXT
GSE137473_gene_exp.diff_MRP7.vs.MRP8.txt.gz 1.4 Mb (ftp)(http) TXT
GSE137473_genes.fpkm_table.MRP7.vs.MRP8.txt.gz 465.1 Kb (ftp)(http) TXT
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