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| Status |
Public on Jul 22, 2020 |
| Title |
Analysis of transposable element expression in mouse ESCs with and without Ncor1, and a time course RNA-seq of mouse ESC differentiation to EpiLCs |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Mouse embryonic stem cells (ESCs) were differentiated to Epiblast-like cells (EpiLCs) according to the protocol in Hayashi et al., 2011. Samples were taken at 0, 12, 24, 36 and 48 hours. Wildtype and Ncor2 knockout ESCs were also sequenced to measure gene expression.
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| Overall design |
The data was sequenced to a depth suitable for the analyiss of transposable elements. All samples have at least 10 million paired-end sequence tags, and each read is 150 bp in length
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| |
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| Contributor(s) |
Hutchins AP, Sun L, Zhuang Q |
| Citation(s) |
33033262 |
| |
| Submission date |
Sep 18, 2019 |
| Last update date |
Oct 21, 2020 |
| Contact name |
Andrew P Hutchins |
| E-mail(s) |
andrewh@sustech.edu.cn
|
| Organization name |
Southern University of Science and Technology
|
| Department |
Bioinformatics
|
| Lab |
Bioinformatics and Genomics
|
| Street address |
1088 Xueyuan Rd
|
| City |
Shenzhen |
| ZIP/Postal code |
518055 |
| Country |
China |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA566099 |
| SRA |
SRP222273 |
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