|
| Status |
Public on Dec 20, 2019 |
| Title |
Determination of ErfA regulon by DAP-seq in several Pseudomonas species |
| Organisms |
Pseudomonas aeruginosa; Pseudomonas putida; Pseudomonas protegens; Pseudomonas chlororaphis |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
ErfA is a transcription factor of Pseudomonas aeruginosa. We here define the genome-wide binding sites of ErfA by DAP-seq in Pseudomonas aeruginosa PAO1 and IHMA87, Pseudomonas chlororaphis PA23, Pseudomonas protegens CHA0 and Pseudomonas putida KT2440.
|
| |
|
| Overall design |
DAP-seq analysis of His-tagged purified ErfA protein binding to sheared genomic DNA of Pseudomonas aeruginosa PAO1 and IHMA87, Pseudomonas chlororaphis PA23, Pseudomonas protegens CHA0 and Pseudomonas putida KT2440.
DAP-seq was performed with ErfA protein from P. aeruginosa or P. chlororaphis. Samples with P. chloroaphis ErfA include "chlo" in the sample title.
|
| |
|
| Contributor(s) |
Trouillon J, Attrée I, Elsen S |
| Citation(s) |
31925438 |
| |
| Submission date |
Sep 18, 2019 |
| Last update date |
Mar 16, 2020 |
| Contact name |
Julian Trouillon |
| Organization name |
ETH Zurich
|
| Department |
Institute of Molecular Systems Biology
|
| Street address |
Otto-Stern-Weg 3
|
| City |
Zurich |
| ZIP/Postal code |
8093 |
| Country |
Switzerland |
| |
|
| Platforms (4)
|
| GPL21297 |
Illumina NextSeq 500 (Pseudomonas aeruginosa) |
| GPL22319 |
Illumina NextSeq 500 (Pseudomonas putida) |
| GPL27478 |
Illumina NextSeq 500 (Pseudomonas chlororaphis) |
| GPL27479 |
Illumina NextSeq 500 (Pseudomonas protegens) |
|
| Samples (36)
|
|
| Relations |
| BioProject |
PRJNA566143 |
| SRA |
SRP222320 |
Less...
More...