|
| Status |
Public on Sep 19, 2019 |
| Title |
Enhancer activation by FGF signalling during otic induction |
| Organism |
Gallus gallus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We report that FGF exposure of sensory progenitors (pPPR) leads to rapid deposition of active chromatin marks H3K27ac near hundreds of FGF-responsive, otic-epibranchial progenitor (OEP) genes, while H3K27ac is depleted in the vicinity of non-otic genes. Genomic regions that gain H3K27ac act as cis-regulatory elements controlling OEP gene expression in time and space and define a unique transcription factor signature likely to control their activity. Thus, during ear induction FGF signalling modifies the chromatin landscape to promote enhancer activation and chromatin accessibility.
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| |
|
| Overall design |
Examination of 2 different histone modifications in Cnt and FGF2 treated posterior pre-placodal (pPPR) explants.
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| |
|
| Contributor(s) |
Tambalo M, Anwar M, Ahmed M, Streit A |
| Citation(s) |
31539539 |
| |
| Submission date |
Sep 18, 2019 |
| Last update date |
Dec 20, 2019 |
| Contact name |
Andrea Streit |
| E-mail(s) |
andrea.streit@kcl.ac.uk
|
| Organization name |
Kings College London
|
| Department |
Centre for Craniofacial & Regenerative Biology
|
| Lab |
Streit
|
| Street address |
Centre for Craniofacial & Regenerative Biology, King's College London
|
| City |
London |
| ZIP/Postal code |
SE1 9RT |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL10223 |
Illumina Genome Analyzer (Gallus gallus) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA566175 |
| SRA |
SRP222347 |
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