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| Status |
Public on Jul 14, 2021 |
| Title |
Effects of Aflibercept administration at 25 mg per kg body weight in retinas of mice subjected to the oxygen-induced retinopathy protocol [P19] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We aimed at identifying the regulatory network of Aflibercept in the context of pathological neovascularization by using the oxygen-induced retinopathy mouse model as a surrogate of proliferative diabetic retinopathy
Total RNA was collected from entire retinal cups dissected from 19-days old mice subjected to the oxygen-induced retinopathy protocol and subsequently injected with Aflibercept either on postnatal days 13 and 15 (OIR+AFLx2) or on postnatal days 13, 15 and 17. Non-injected OIR littermates (OIR) and age-matched mice housed in normoxic conditions (normoxia) were used as controls.
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| Overall design |
Four treatments were considered, namely normoxic controls, OIR mice, OIR mice injected twice with Aflibercep (OIR+AFLx2), and OIR mice injected three times with Aflibercept (OIR+AFLx3). Each group contained three biological replicates, i.e. three mice. All RNA samples were collected on postnatal day 19. Thus, 12 samples were analyzed in total: 1 time-point, 4 treatments, and 3 replicates per treatment. Each sample consists of total RNA isolated from the 2 entire pooled retinas of 1 mouse.
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| Contributor(s) |
Rojo Arias JE, Jászai J |
| Citation(s) |
34321516 |
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| Submission date |
Sep 19, 2019 |
| Last update date |
Aug 03, 2021 |
| Contact name |
Jesus Eduardo Rojo Arias |
| E-mail(s) |
erojoarias@gmail.com
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| Organization name |
University of Cambridge
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| Department |
Wellcome-MRC Cambridge Stem Cell Institute
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| Lab |
Alcolea
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| Street address |
Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, Puddicombe Way
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| City |
Cambridge |
| ZIP/Postal code |
CB2 0AW |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL21877 |
[MTA-1_0] Affymetrix Mouse Transcriptome Array 1.0 |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA566356 |
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