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Series GSE138017 Query DataSets for GSE138017
Status Public on Dec 31, 2020
Title H3K4methylation in Setd1a loss-of-function Raw264.7 cells upon LPS or Dex+LPS stimulation
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Nuclear interaction studies by ChIP coupled with mass spectrometry identified the COMPASS/SETD1A complex as interaction partner of the glucocorticoid receptor (GR) in murine bone marrow-derived macrophages (BMDMs). Here, we profiled H3K4me1, H3K4me2 and H3K4me3 in wild-type and Setd1a hypermorphic (Setd1aDel/+) Raw264.7 cells after LPS and Dex+LPS stimulation by spike-in ChIP-Seq.
 
Overall design We profiled changes in H3K4me1, H3K4me2 and H3K4me3 in wild-type and Setd1aDel/+ (loss-of-function) Raw264.7 cells after challenge with LPS or Dex+LPS. All ChIP-Seq experiments were done in duplicates or more. We used Drosophila melanogaster chromatin as spike-in control.
 
Contributor(s) Greulich F, Uhlenhaut NH
Citation(s) 33567280, 34189474
Submission date Sep 25, 2019
Last update date Jul 08, 2021
Contact name Franziska Greulich
E-mail(s) franziska.greulich@tum.de
Organization name TU München
Department Metabolic Programming
Lab AG Uhlenhaut
Street address Gregor-Mendel-Str. 2
City Freising
ZIP/Postal code 85354
Country Germany
 
Platforms (3)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (48)
GSM4096673 GAR0965: Raw264.7_wt_DexLPS_aGR_rep1
GSM4096674 GAR2146: Raw264.7_wt_DexLPS_aGR_rep2
GSM4096675 GAR2148: Raw264.7_wt_DexLPS_aGR_rep3
Relations
BioProject PRJNA574157
SRA SRP223229

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE138017_RAW.tar 16.0 Gb (http)(custom) TAR (of BW, TAB, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
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