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| Status |
Public on Sep 28, 2019 |
| Title |
Photoreceptor Otx2 cKO |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Homeobox transcription factor Otx2 is a key actor in retinal development, but in adult stage its function is not yet elucidated. In order to elucidate it we induced Otx2 KO specifically in MATURE PHOTORECEPTORS. Here, we report RNA seq data, that were produced in order to investigate on the effect of Otx2 ablation on its regulatoy network. Post natal day 30 (P30) is the time of KO induction. KO mice were collected 2, 4 and 8 days after Otx2 ablation.
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| Overall design |
KO was induced with single tamoxifen injection at P30 and mice collected 2, 4 and 8 days after KO. Single eye from each mouse was collected, retina dissected and quickly RNA was extracted, validated for the quality and sent to the RNAseq platform. 3 mice for each condition were used. The other eye was used in parallel to validate Otx2 KO by immunostaining.
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| Contributor(s) |
Lamonerie T, Pensieri P, Mantilleri A |
| Citation(s) |
34475267 |
| |
| Submission date |
Sep 27, 2019 |
| Last update date |
Sep 13, 2021 |
| Contact name |
PASQUALE PENSIERI |
| E-mail(s) |
ppensieri@unice.fr
|
| Phone |
+33603960714
|
| Organization name |
INSTITUT BIOLOGIE VALROSE
|
| Department |
SCIENCES NATURELLES
|
| Lab |
NEURODEVELOPMENT , LAMONERIE'S TEAM
|
| Street address |
PARC VALROSE
|
| City |
NICE |
| ZIP/Postal code |
06108 |
| Country |
France |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA574601 |
| SRA |
SRP223505 |
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