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Series GSE138121 Query DataSets for GSE138121
Status Public on Oct 21, 2020
Title scRNAseq of human iPSC-derived motor neurons from control and ALS patients.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We compare single cell transcriptomic profiles of motor neurons differentiated in vitro from human induced pluripotent stem cells derived from control and ALS patients.
 
Overall design Human induced pluripotent stem cell-derived motor neurons were differentiated in vitro over 18 days following a previously described protocol (Maury et al. Nat Biotechnol, 2015). Cells were processed for single cell sequencing following the manufacturer's guidelines for the 10X Genomics Chromium or Illumina/Bio-Rad ddSEQ platforms. A total of 25 samples were processed in 6 batches. Replicates of cell lines CS83iCTR-33n1, CS0179iCTR-n1, CS2XWCiALS-n1, CS29iALS-C9n1, CS52iALS-C9n6, CS25iCTR-18n2, and CS29iALS-C9n1.ISO were processed across multiple batches.
 
Contributor(s) Svendsen CN, Ho R, Workman MJ
Citation(s) 33382996
https://doi.org/10.1101/2020.04.27.064584
Submission date Sep 27, 2019
Last update date Sep 29, 2021
Contact name Michael Workman
E-mail(s) michael.workman@cshs.org
Organization name Cedars-Sinai Medical Center
Street address 8700 Beverly Blvd., AHSP Suite 8405
City Los Angeles
State/province CA
ZIP/Postal code 90048
Country USA
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (25)
GSM4100677 BatchA_CTR_0083_d00
GSM4100678 BatchA_CTR_0083_d06
GSM4100679 BatchA_CTR_0083_d12
Relations
BioProject PRJNA574650
SRA SRP223536

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE138121_BatchA_ddSEQ_UMIcounts.csv.gz 14.4 Mb (ftp)(http) CSV
GSE138121_BatchB1_ddSEQ_UMIcounts.csv.gz 734.2 Kb (ftp)(http) CSV
GSE138121_BatchB2_ddSEQ_UMIcounts.csv.gz 5.9 Mb (ftp)(http) CSV
GSE138121_BatchC_ddSEQ_UMIcounts.csv.gz 13.4 Mb (ftp)(http) CSV
GSE138121_BatchD_ddSEQ_UMIcounts.csv.gz 15.6 Mb (ftp)(http) CSV
GSE138121_BatchE_10X_UMIcounts.csv.gz 9.8 Mb (ftp)(http) CSV
GSE138121_BatchF_10X_UMIcounts.csv.gz 12.0 Mb (ftp)(http) CSV
GSE138121_CellBarcodes.csv.gz 121.2 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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