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| Status |
Public on Jul 01, 2020 |
| Title |
VRK-1 Extends Lifespan by Activation of AMPK via Phosphorylation |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Vaccinia virus-related kinase (VRK) is an evolutionarily conserved nuclear protein kinase. VRK-1, the single Caenorhabditis elegans VRK ortholog, functions in cell division and germline proliferation. However, the role of VRK-1 in post-mitotic cells and adult lifespan remain unknown. Here, we show that VRK-1 increases organismal longevity by activating the cellular energy sensor, AMP-activated protein kinase (AMPK), via direct phosphorylation. We found that overexpression of vrk-1 in the soma of adult C. elegans increased lifespan, and conversely, inhibition of vrk-1 decreased lifespan. In addition, vrk-1 was required for longevity conferred by mutations that inhibit C. elegans mitochondrial respiration, which requires AMPK. Notably, VRK-1 directly phosphorylated and up-regulated AMPK in both C. elegans and cultured human cells. Thus, our data show that the somatic nuclear kinase, VRK-1, promotes longevity through AMPK activation, and this function appears to be conserved between C. elegans and humans.
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| Overall design |
The investigation of the changes of gene expression in vrk-1 RNAi-treated wild-type or isp-1(qm150) mutant animals
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| Contributor(s) |
Park S, Artan M, Han S, Lee SV |
| Citation(s) |
32937443 |
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| Submission date |
Sep 29, 2019 |
| Last update date |
Oct 01, 2020 |
| Contact name |
Seung-Jae V Lee |
| E-mail(s) |
seungjaevlee@kaist.ac.kr
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| Organization name |
KAIST
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| Department |
Life Sciences
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| Lab |
Room 4203
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| Street address |
291 Daehak-ro
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| City |
Daejeon |
| ZIP/Postal code |
34141 |
| Country |
South Korea |
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| Platforms (1) |
| GPL18245 |
Illumina HiSeq 2500 (Caenorhabditis elegans) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA574844 |
| SRA |
SRP223634 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE138129_RAW.tar |
1.9 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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