|
| Status |
Public on May 31, 2021 |
| Title |
Microbes Malnutrition Microglia |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
This study examines the role of early exposure to gut microbes and poor diet on microglial function in mice. Groups = control (CON), malnourished (MAL), and malnourished + microbial exposure (E/MALBG). CD11b+ cells (microglial enrichment) were isolated from whole mouse brains (Adult Brain Disruption Kit, Miltenyi Biotec). After sample quality control (Agilent 2100 Bioanalyzer), qualifying samples were sent for RNA-Seq (Illumina NextSeq 500 with Paired End 42bp × 42bp reads; demultiplexed: Illumina's bcl2fastq2). Following alignment against mouse reference genes (STAR aligner), DEG analyses was conducted using the DESeq2 pipeline.
|
| |
|
| Overall design |
RNA-Seq from Cd11b+ brain cells
|
| |
|
| Contributor(s) |
Bauer KC, Finlay BB, Lee A, Hancock RE |
| Citation(s) |
35019164 |
| |
| Submission date |
Sep 30, 2019 |
| Last update date |
Aug 02, 2022 |
| Contact name |
Kylynda C Bauer |
| E-mail(s) |
Kylynda.c.bauer@gmail.com
|
| Organization name |
Michael Smith Laboratories
|
| Lab |
Finlay Lab
|
| Street address |
#301 – 2185 East Mall
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V6N2Y8 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (16)
|
|
| Relations |
| BioProject |
PRJNA575001 |
| SRA |
SRP223737 |
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