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Series GSE13824 Query DataSets for GSE13824
Status Public on Aug 29, 2009
Title SIV Encephalitis and Uninfected Control: Hippocampus Expression Profiles
Organism Macaca mulatta
Experiment type Expression profiling by array
Summary HIV-associated dementia (HAD) is a syndrome occurring in HIV-infected patients with advanced disease that likely develops as a result of macrophage and microglial activation as well as other immune events triggered by virus in the central nervous system. The most relevant experimental model of HAD, rhesus macaques exhibiting SIV encephalitis (SIVE), closely reproduces the human disease and has been successfully used to advance our understanding of mechanisms underlying HAD. In this study we integrate gene expression data from uninfected and SIV-infected hippocampus with a human protein interaction network and discover modules of genes whose expression patterns distinguish these two states, to facilitate identification of neuronal genes that may contribute to SIVE/HIV cognitive deficits. Using this approach we identify several downregulated candidate genes and select one, EGR1, a key molecule in hippocampus-related learning and memory, for further study. We show that EGR1 is downregulated in SIV-infected hippocampus and that it can be downregulated in differentiated human neuroblastoma cells by treatment with CCL8, a product of activated microglia. Integration of expression data with protein interaction data to discover discriminatory modules of interacting proteins can be usefully employed to prioritize differentially expressed genes for further study. Investigation of EGR1, selected in this manner, indicates that its downregulation in SIVE may occur as a consequence of the host response to infection, leading to deficits in cognition.
 
Overall design RNA from duplicate hippocampal samples taken from nine control monkeys and nine monkeys with evidence of SIV encephalitis were hybridized to Affymetrix arrays.
 
Contributor(s) Head S, Gersten M, Marcondes C, Flynn C, Alirezaei M, Ravasi T, Ideker T, Fox H
Citation(s) 19812322
Submission date Dec 04, 2008
Last update date Jul 18, 2012
Contact name Howard Fox
E-mail(s) hfox@unmc.edu
Phone 402 559-4821
Fax 402 559-7495
Organization name University of Nebraska Medical Center
Department Pharmacology and Experimental Neuroscience
Street address 985800 Nebraska Medical Center - DRC 3008
City Omaha
State/province NE
ZIP/Postal code 68198
Country USA
 
Platforms (1)
GPL3535 [Rhesus] Affymetrix Rhesus Macaque Genome Array
Samples (36)
GSM347800 SIVE, biological replicate 1, technical replicate 1
GSM347801 SIVE, biological replicate 1, technical replicate 2
GSM347802 Control, biological replicate 1, technical replicate 1
Relations
BioProject PRJNA110495

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Supplementary file Size Download File type/resource
GSE13824_RAW.tar 288.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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