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| Status |
Public on Oct 08, 2019 |
| Title |
Transcriptomic analysis to identify the putative genes under the regulation of two component system BtrK/BtrR in C. acetobutylicum |
| Organism |
Clostridium acetobutylicum |
| Experiment type |
Expression profiling by array
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| Summary |
To identify the putative genes under the regulation of two component system BtrK/BtrR, we performed a comparative transcriptomic analysis of the BtrK/BtrR overexpressing strain and the contron strain. Finally, we found that BtrK/BtrR was a global regulator and exerted pleiotropic regulatory role in C. acetobutylicum
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| Overall design |
Samples for RNA extraction and microarray analysis were collected after 24 and 48 h of fermentation. From this analysis, 691 and 424 genes exhibited significantly altered transcription (fold change ≥ 2.0) after the overexpression of btrK/btrR at 24 and 48 h, respectively. Among these genes, 153 genes showed significantly different expression at both 24 and 48 h.
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| Contributor(s) |
Yang Y |
| Citation missing |
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| Submission date |
Oct 05, 2019 |
| Last update date |
Oct 10, 2019 |
| Contact name |
Yunpeng Yang |
| E-mail(s) |
ypyang01@sibs.ac.cn
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| Organization name |
Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences
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| Street address |
300， Fenglin Road
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| City |
shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
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| Platforms (1) |
| GPL27577 |
Agilent-024359 Agilent SurePrint C. acetobutylicum Gene Expression Microarray 8x60K |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA576001 |
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