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| Status |
Public on Apr 13, 2020 |
| Title |
The chromatin remodeling enzyme Chd4 regulates genome architecture in the mouse brain |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Other
Expression profiling by high throughput sequencing
|
| Summary |
The development and function of the brain require tight control of gene expression. Genome architecture is thought to play a critical regulatory role in gene expression, but the mechanisms governing genome architecture in the brain in vivo remain poorly understood. Here, we report that conditional knockout of the chromatin remodeling enzyme Chd4 in granule neurons of the mouse cerebellum increases accessibility of gene regulatory sites genome-wide in vivo. Conditional knockout of Chd4 promotes recruitment of the architectural protein complex cohesin preferentially to gene enhancers in granule neurons in vivo. Importantly, in vivo profiling of genome architecture reveals that conditional knockout of Chd4 strengthens interactions among developmentally repressed contact domains as well as genomic loops in a manner that tightly correlates with increased accessibility, enhancer activity, and cohesin occupancy at these sites. Collectively, our findings define a role for chromatin remodeling in the control of genome architecture organization in the mammalian brain.
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| Overall design |
Cerebella were dissected from P22 mice. For ChIP-seq, protein-DNA complexes were isolated from sonicated lysates. For Hi-C, nuclei were digested and blunt-end ligated in the presence of a biotinylated nucleotide. Ligation complexes were then isolated from sonicated DNA by streptavidin pull-down. For DNaseI-seq, nuclei were digested with DNaseI, then DNA under 500bp were selected. For nuclear RNA-seq, total RNA was extracted from nuclei.
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| Web link |
https://www.nature.com/articles/s41467-020-17065-z
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| Contributor(s) |
Goodman JV, Yamada T, Yang Y, Kong L, Wu DY, Zhao G, Gabel HW, Bonni A |
| Citation(s) |
32647123 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 NS041021 |
SIGNAL TRANSDUCTION PATHWAYS REGULATING NEURON DIFFERENTIATION |
WASHINGTON UNIVERSITY |
AZAD BONNI |
| R01 MH117405 |
MECHANISMS OF EPIGENETIC REGULATION IN NERVOUS SYSTEM DEVELOPMENT |
WASHINGTON UNIVERSITY |
Harrison W Gabel |
| F30 HD094447 |
Elucidating the Roles of Mbd3 during Cerebellar Development |
WASHINGTON UNIVERSITY |
Jared Vega Goodman |
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| |
| Submission date |
Oct 14, 2019 |
| Last update date |
Jul 13, 2020 |
| Contact name |
Jared V Goodman |
| E-mail(s) |
jvgoodman@wustl.edu
|
| Organization name |
Washington University in St. Louis School of Medicine
|
| Street address |
4523 Clayton Ave
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (38)
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| Relations |
| BioProject |
PRJNA577452 |
| SRA |
SRP225547 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE138822_RAW.tar |
13.0 Gb |
(http)(custom) |
TAR (of HIC) |
| GSE138822_chd4DhsDiffBindDeSeq2ReportAll.txt.gz |
2.6 Mb |
(ftp)(http) |
TXT |
| GSE138822_compartmentalDomains.bedpe.gz |
56.5 Kb |
(ftp)(http) |
BEDPE |
| GSE138822_ctcfChd4Ko.bigwig |
427.9 Mb |
(ftp)(http) |
BIGWIG |
| GSE138822_ctcfChd4Wt.bigwig |
438.2 Mb |
(ftp)(http) |
BIGWIG |
| GSE138822_dhsNonProm.bed.gz |
2.4 Mb |
(ftp)(http) |
BED |
| GSE138822_dhsNonProm.overlappingH3K27ac.bed.gz |
768.2 Kb |
(ftp)(http) |
BED |
| GSE138822_dhsNonProm.overlappingH3K4me1.bed.gz |
784.6 Kb |
(ftp)(http) |
BED |
| GSE138822_dhsTss.bed.gz |
300.5 Kb |
(ftp)(http) |
BED |
| GSE138822_dhsTss.overlappingH3K27ac.bed.gz |
233.2 Kb |
(ftp)(http) |
BED |
| GSE138822_dnaseiChd4Ko.bigwig |
991.8 Mb |
(ftp)(http) |
BIGWIG |
| GSE138822_dnaseiChd4Wt.bigwig |
1.2 Gb |
(ftp)(http) |
BIGWIG |
| GSE138822_h3k27acP60Chd4Ko.bigwig |
567.4 Mb |
(ftp)(http) |
BIGWIG |
| GSE138822_h3k27acP60Chd4Wt.bigwig |
583.3 Mb |
(ftp)(http) |
BIGWIG |
| GSE138822_h3k4me1Chd4Ko.bigwig |
493.0 Mb |
(ftp)(http) |
BIGWIG |
| GSE138822_h3k4me1Chd4Wt.bigwig |
489.5 Mb |
(ftp)(http) |
BIGWIG |
| GSE138822_koCombined.hic |
5.3 Gb |
(ftp)(http) |
HIC |
| GSE138822_loopDomains.bedpe.gz |
33.6 Kb |
(ftp)(http) |
BEDPE |
| GSE138822_loopsWtKoMerged.10kb.bedpe.gz |
167.5 Kb |
(ftp)(http) |
BEDPE |
| GSE138822_nucRNA_eRNAs_condition_treated_results.csv.gz |
601.7 Kb |
(ftp)(http) |
CSV |
| GSE138822_smc1Chd4Ko.bigwig |
330.5 Mb |
(ftp)(http) |
BIGWIG |
| GSE138822_smc1Chd4Wt.bigwig |
359.1 Mb |
(ftp)(http) |
BIGWIG |
| GSE138822_wtCombined.hic |
4.5 Gb |
(ftp)(http) |
HIC |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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