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| Status |
Public on Oct 30, 2019 |
| Title |
Signaling protein antibody microarray analyses for islets of control and IFT88 knockout mice [SET100] |
| Platform organism |
Homo sapiens |
| Sample organism |
Mus musculus |
| Experiment type |
Protein profiling by protein array
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| Summary |
β-cell specific IFT88 knock-out mice recapitulate human diabetes with impaired insulin secretion and altered islet hormone paracrine regulation. To examine the signaling pathways regulating islet cell function, we subjected protein lysates of whole islets from control and IFT88 knockout mice to a commercial signaling-protein array analysis (Full Moon Bio, Inc).
Samples were probed against 1358 antibodies with 2 replicates per antibody on 76 x 25 x 1mm glass slides.
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| Overall design |
IFT88 fl/fl mice and INS1-Cre mice on a C57BL/6 background were crossed to generate β-cell specific IFT88 knockout mice. Animals were weaned at age 3 weeks and maintained on a standard chow diet. Islets were isolated using modified Lacy protocol and allowed to recover in culture overnight before collection and lysis. Total protein lysates were prepared from 250 size-matched islets per sample (2 controls and 2 knockouts, each pooled from 2 mice) and used for FullMoonBio Signaling Explorer Array (SEA100) analysis.
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| Contributor(s) |
Hughes J |
| Citation(s) |
32253320 |
| |
| Submission date |
Oct 15, 2019 |
| Last update date |
May 08, 2020 |
| Contact name |
Jing Hughes |
| E-mail(s) |
jing.hughes@gmail.com
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| Phone |
314-273-1098
|
| Organization name |
Washington University
|
| Department |
Internal Medicine
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| Lab |
Hughes
|
| Street address |
660 S Euclid
|
| City |
St Louis |
| State/province |
Missouri |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
| GPL27610 |
Signaling Explorer Array (SET100) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA577686 |
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