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Series GSE139034 Query DataSets for GSE139034
Status Public on Dec 10, 2019
Title Sex chromsomes affect gene expression of healthy CD4+ T cells in B6 gonadal males
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To determine the role of sex chromsomes in sex differences of autoimmune disease, we used high throughput RNA sequencing to analyze the transciptomes of CD4+ T cells stimuated with antiCD3/CD28 from XXSry and XY-Sry mice using the “four core genotypes” mouse model. We found higher expression of several X genes in the XY- genotype compared to XX, suggesting a difference in DNA methylation of these genes between Xp and Xm. In a hypothesis driven targeted approach, we investigated two immunomodulatory X genes, Tlr7 and FoxP3 and found that the promoter regions of both genes had more DNA methylation on the Xp than Xm, and that both genes had higher expression in autoantigen stimulated CD4+ T cells from XY- compared to XX mice. This study provides new insights regarding how sex chromsomes affect gene expression and cause sex differences in autoimmune disease.
Overall design Male mice from the "four core genotypes" mouse model (XXSry and XY-Sry) were gonadectomized at 4-6 weeks old. At 8-12 weeks old, auxillary, brachial, and inguinal draining lymph nodes were collected and processed into single cell suspensions and sorted for CD4+ T cells using the EasySep Mouse CD4+ T cell isolation kit (STEMCELL). Cells were stiumulated with anti-CD3/CD28 Dyna Beads for 36 hours and RNA was isolated for analysis by high throughput RNA sequencing.
Contributor(s) Golden L, Itoh Y, Itoh N, Voskuhl R
Citation(s) 31822606
Submission date Oct 17, 2019
Last update date Jan 08, 2020
Contact name Yuichiro Itoh
Phone 3102068999
Organization name UCLA
Department Neurology
Street address 635 Charles E. Young Drive South
City Los Angeles
State/province California
ZIP/Postal code 90095
Country USA
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (10)
GSM4127846 XXSry1_B6
GSM4127847 XXSry2_B6
GSM4127848 XXSry3_B6
BioProject PRJNA578120
SRA SRP226075

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Supplementary file Size Download File type/resource
GSE139034_mm10.gene_B6CD4M_ensGene.xlsx 2.0 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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