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| Status |
Public on Dec 09, 2009 |
| Title |
NK-cell associated receptors expression in EBV-positive gamma-delta T cell lines. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The contribution of chronic antigen stimulation to the occurrence of lymphoproliferative disorder (LPD) with the gamma-delta T-cell lineage is unclear, despite the fact that Epstein-Barr virus (EBV) positive T-cell LPD is derived from antigen-stimulated cytotoxic T-cells. Given the possible association of antigen stimulation with the development of cytotoxic T-cell LPD, we compared gene expression patterns in Epstein-Barr virus (EBV)-positive gamma-delta T-cell lines derived from patients with nasal T-cell lymphoma and chronic active EBV infection and those in gamma-delta T-cells from healthy volunteers. Three EBV-positive gamma-delta T-cells lines, SNT cells (SNT-8, SNT-13 and SNT-15), were used in this study. SNT-8 was established from patients with nasal T-cell lymphoma and SNT-13, -15 were established from patients with chronic active EBV infection (Zhang Y, et al., Br J Cancer 94:599-608, 2006). All the SNT cells exhibits common rearrangement of Vgamma9-JgammaP and Jdelta3 genes. The gamma-delta T-cells obtained from healthy volunteers were expanded ex vivo by 1 microM of zoledronate (ZOL) plus IL-2 for 14 days incubation.
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| Overall design |
We compared gene expression profiling in 3 EBV-positive positive gamma-delta T-cells lines with those in gamma-delta T cells obtaied from a healthy volunteer. Global gene expression was analyzed using the Affymetrix Human Genome U133 2.0 Plus GeneChip Set. Analysis of variance (ANOVA) was done using GeneSifter® (VizXLabs). Values of P<0.05 were considered to be a statistically significant difference.
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| Contributor(s) |
Zhang Y, Ohyashiki JH, Shimizu N, Ohyashiki K |
| Citation(s) |
20132662 |
| |
| Submission date |
Dec 10, 2008 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Junko H Ohyashiki |
| E-mail(s) |
junko@hh.iij4u.or.jp
|
| Phone |
+81-3-3342-6111(ext.5999)
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| Fax |
+81-3-5381-6651
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| Organization name |
Tokyo Medical University
|
| Department |
Intractable Immune System Diseases Research Center
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| Street address |
6-7-1, Nishishinjuku, Shinjuku-ku
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| City |
Tokyo |
| ZIP/Postal code |
160-0023 |
| Country |
Japan |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA110321 |
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