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| Status |
Public on Jun 19, 2020 |
| Title |
Single cell RNA-seq revealed different cell types induced by loss of ARID1A in undifferentiated and differentiation (day 10) H9 hESCs. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We reported loss of ARID1A promoted neurogenesis and inhibited cardiogenesis. Under microscopy, we observed that spontaneously differentiated cells were induced in ARID1A KO H9 hESCs cultured in mTesR medium. After cardiac differentiation for 10 days, we also observed the cell types were totally different between WT and ARID1A KO cells. We did not know what cells types were. Here scRNA-seq were used to identify the cell types in WT H9 hESCs and ARID1A KO H9 hESCs.
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| Overall design |
Identification of different cell types induced by loss of ARID1A in undifferentiated and differentiated H9 hESCs.
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| |
|
| Contributor(s) |
Yang L, Liu J |
| Citation(s) |
32646524 |
| |
| Submission date |
Oct 24, 2019 |
| Last update date |
Jul 16, 2020 |
| Contact name |
Lei Yang |
| E-mail(s) |
lyang7@iu.edu
|
| Phone |
(317) 278-5233
|
| Organization name |
Indiana University School of Medicine
|
| Department |
Department of Pediatrics
|
| Street address |
1044 W. Walnut St.
|
| City |
Indianapolis |
| State/province |
Indiana |
| ZIP/Postal code |
46202 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE139343 |
Essential and Opposite Roles of ARID1A in Coordinating Human Cardiogenesis and Neurogenesis from Pluripotent Stem Cells |
|
| Relations |
| BioProject |
PRJNA579351 |
| SRA |
SRP226855 |
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