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| Status |
Public on Jul 07, 2020 |
| Title |
Effect of pre-exposure prophylaxis with tenofovir disoproxil fumarate and emtricitabine on gene expression in the duodenum |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Pre-exposure prophylaxis (PrEP) with tenofovir disoproxil fumarate (TDF) and emtricitabine (FTC) effectively prevents infection with HIV-1. We sought to determine how use of these medications affects gene expression. We performed a small, prospective trial (https://clinicaltrials.gov/ct2/show/NCT02621242) of eight men initiating PrEP with TDF/FTC. We obtained duodenal biopsies before treatment initiation and after two months of daily use. We isolated RNA from duodenal biopsies preserved with RNAlater. Gene expression was measured from all eight men at both time points.
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| Overall design |
Gene expression was measured by Illumina HumanHT-12 v4 Expression BeadChips. Samples were paired with samples taken at baseline (pre-treatment) and after two months of daily oral use. Duodenal biopsies were preserved with RNAlater at -80 degrees C. Samples were not replicated on the arrays.
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| Contributor(s) |
Hladik F, Levy CN, Hughes SM |
| Citation(s) |
33015651 |
| |
| Submission date |
Oct 30, 2019 |
| Last update date |
Oct 13, 2020 |
| Contact name |
Sean Hughes |
| E-mail(s) |
smhughes@uw.edu
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| Organization name |
University of Washington
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| Street address |
850 Republican St
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE139611 |
Effect of pre-exposure prophylaxis with tenofovir disoproxil fumarate and emtricitabine on gene expression |
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| Relations |
| BioProject |
PRJNA580487 |
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