|
| Status |
Public on Mar 25, 2020 |
| Title |
Intracellular Staphylococcus aureus persisters upon antibiotic exposure |
| Organism |
Staphylococcus aureus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Bacterial persister cells are phenotypic variants that exhibit a transient non-growing state and antibiotic tolerance. Here we provide in vitro evidence of Staphylococcus aureus persisters within infected host cells. We show that the bacteria surviving antibiotic treatment within host cells are persisters, displaying biphasic killing and reaching a uniformly non-responsive, non-dividing state when followed at the single-cell level. This phenotype is stable but reversible upon antibiotic removal. Intracellular S. aureus persisters remain metabolically active, but display an altered transcriptomic profile consistent with activation of stress responses, including the stringent response as well as cell-wall stress, SOS and heat-shock responses. These changes are associated with multidrug tolerance after exposure to a single antibiotic. We hypothesize that intracellular S. aureus persisters may constitute a reservoir for relapsing infection, and could contribute to therapeutic failures.
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| |
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| Overall design |
3 Intracellular persister replicates and 3 control replicates
|
| |
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| Contributor(s) |
Peyrusson F, Varet H, Legendre R, Sismeiro O, Coppée J, Wolz C, Tenson T, Van Bambeke F |
| Citation(s) |
32366839 |
| |
| Submission date |
Oct 31, 2019 |
| Last update date |
May 12, 2020 |
| Contact name |
Rachel Legendre |
| E-mail(s) |
rachel.legendre@pasteur.fr
|
| Organization name |
Institut Pasteur
|
| Department |
Research and Resource Center for Scientific Informatics
|
| Lab |
Hub of Bioinformatics and Biostatistics
|
| Street address |
28, rue du docteur Roux
|
| City |
Paris |
| ZIP/Postal code |
75724 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL19006 |
Illumina HiSeq 2500 (Staphylococcus aureus) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA586837 |
| SRA |
SRP227811 |
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