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Series GSE13998 Query DataSets for GSE13998
Status Public on Dec 15, 2009
Title Transcriptional analysis of RelE family toxin overexpression in Mycobacterium tuberculosis
Organism Mycobacterium tuberculosis
Experiment type Expression profiling by array
Summary To understand the mechanism of action of the RelE family of toxins on metabolism of Mycobacterium tuberculosis. To this effect, toxin expression was induced under control of a tetracycline regulatable promoter and transcriptional profiles compared during overexpression of the three RelE toxin family members. Profiles were also compared to previously published profiles of drugs of known mechanism of action against this organism.
 
Overall design For the design, RNA isolated from cells where toxin expression was induced with anhydrotetracycline was converted to Cy5-labeled cDNA whereas RNA derived from cells in which toxin expression was not induced (treated with ethanol solvent only) was converted to Cy3-labeled cDNA. Time points were taken for each overexpression condition. Treatments were performed indepedently a minimum of two times to give independent biological replicates.
 
Citation(s) 20061486
Submission date Dec 16, 2008
Last update date Mar 20, 2012
Contact name Helena I Boshoff
E-mail(s) hboshoff@niaid.nih.gov
Phone 301-451-9438
Fax 301-402-0993
Organization name NIAID/ NIH
Department LIG
Lab Tuberculosis Research Section
Street address 12441 Parklawn Drive
City Rockville
State/province MD
ZIP/Postal code 20852
Country USA
 
Platforms (1)
GPL1343 NIAID Mycobacterium tuberculosis microarray Mtbb
Samples (32)
GSM351644 MTb-relE1 - 6 hr - repeat 1 - mAdbID:77666
GSM351645 MTb-relE2 - 6 hr - repeat 2 - mAdbID:77667
GSM351646 MTb-relE3 -6 hr - repeat 2 - mAdbID:77668
Relations
BioProject PRJNA112497

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE13998_RAW.tar 13.3 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table
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