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| Status |
Public on Dec 19, 2019 |
| Title |
Transcriptomic analysis of Drosophila olfactory projection neurons |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Molecular interactions at the cellular interface mediate organized assembly of single cells into tissues, and thus govern the development and physiology of multicellular organisms. Here, we developed a cell-type-specific, spatiotemporally-resolved approach to profile cell surface proteomes in intact tissues. Quantitative profiling of cell-surface proteomes of Drosophila olfactory projection neurons (PNs) in pupae and adults revealed a global downregulation of wiring molecules and an up-regulation of synaptic molecules in the transition from developing to mature PNs. To compare the RNA and protein dynamics of PN surface molecules in the developing-to-mature transition, we also profiled the PN transcriptomes from 36hAPF pupae and 5d adults. We performed bulk RNA-sequencing of PNs with 3 samples for each stage and 2k cells for each sample.
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| Overall design |
6 samples, 3 from development and 3 from adult
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| |
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| Contributor(s) |
Li H, Li J |
| Citation missing |
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| |
| Submission date |
Nov 07, 2019 |
| Last update date |
Dec 19, 2019 |
| Contact name |
Hongjie Li |
| E-mail(s) |
hongjie.li@bcm.edu
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| Organization name |
Baylor College of Medicine
|
| Street address |
one Baylor Plaza
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA588248 |
| SRA |
SRP229094 |
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