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| Status |
Public on Nov 09, 2019 |
| Title |
These are male and female mus musculus mRNA expression profiles from whole liver tissue of fatp2 knock-out and wildtype mice. . |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Fatty acid transport protein 2 (FATP2) is highly expressed in liver, small intestine, and kidney where it functions in both the uptake of exogenous long chain fatty acids (LCFAs) and in the activation to CoA thioesters of very long chain fatty acids (VLCFAs). Here we address the phenotypic impacts of deleting FATP2 followed by an unbiased RNA-seq analysis of the liver transcriptome. Wild type (C57BL/6J) and fatp2 null (fatp2-/-) mice (5 weeks old) were maintained on a standard chow diet for 6 weeks (11 weeks old). The male fatp2-/- mice had 258 differentially expressed genes (DEGs) and the female mice had a total of 91. Of significance was the finding that most of the genes with increased expression in the fatp2-/- liver are regulated by the transcription factor peroxisome proliferator-activated receptor alpha (PPARĪ±). Taken together, FATP2 has a broad impact on the expression of key lipid metabolic genes in the liver regulated by PPARĪ±.
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| Overall design |
For RNA-sequencing, we used 4 animals for each experimental group, and we had 4 experimental groups. The groups were - [1] wildtype c57bl6 male mice, [2] wildtype c57bl6 female mice, [3] fatp2 knock-out c57bl6 male mice, and [4] fatp2 knock-out c57bl6 female mice.
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| Contributor(s) |
Perez VM, Gabell JT, Behrens MR, DiRusso CC, Black PN |
| Citation(s) |
32188695 |
| |
| Submission date |
Nov 08, 2019 |
| Last update date |
Mar 24, 2020 |
| Contact name |
Vincent Michael Perez |
| E-mail(s) |
vincentmichaelperez@gmail.com
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| Phone |
4172883742
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| Organization name |
Tempus AI
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| Department |
Translational reasearch
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| Street address |
25 Alexandria Wy
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27703 |
| Country |
USA |
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| Platforms (1) |
| GPL27637 |
Illumina Novaseq 6000 (Mus musculus) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA588426 |
| SRA |
SRP229353 |
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